Journal: Vaccine

Article Title: Kunjin replicon-based simian immunodeficiency virus gag vaccines

doi: 10.1016/j.vaccine.2008.04.001

Figure Lengend Snippet: Challenge of Kunjin SIV gag VLP vaccinated mice with A20 cells expressing SIV mac239 Gag. (A) Western blot of A20-EGFP-SIV gag and parental A20 cells with anti-SIV Gag antibody (55-2F12) and anti-GFP antibody. 15 μg of total protein was loaded in each well. (B) Kaplan Meier plot of survival. Groups of mice ( n = 4–6) were immunised twice with the indicated VLP vaccines and were then challenged ≈9 months later with A20-EGFP-SIV gag cells. Animals were euthanased when tumours reached 100 mm 2 . (C) Mean growth curves for the tumours from the same experiment. When an animal was euthansed a value of 100 mm 2 was included for that animal in the mean for all subsequent time points.

Article Snippet: This plasmid was used to transfect the BALB/c-derived A20 murine lymphoblastic leukemia cells (ATCC, TIB-208) using the GeneJammer Transfection Reagent (StrataGene, La Jolla, CA, USA).

Techniques: Expressing, Western Blot